Part:BBa_K2220010:Design

Secrete FliC-eGFP fusion protein

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 286
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1785
  Illegal XhoI site found at 244
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 595
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We have done the codon optimization.

Source

eGFP comes from other iGEM team; FliC comes from E.coli K12; alpha-factor comes from pyes2/ct/alpha-factor plasmid.